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Frequently Asked Questions are available for this Product. Components 15g/L Agar 10g/L Tryptone 5 g/L Yeast Extract 5 g/L NaCl Features and Benefits Lennox EZMix ™ powder with agar provides: • A budget-friendly alternative to pre-poured plates • Granulated, dust-free format for safer handling and faster mixing • Convenient small package to eliminate weighing • Easy scale-up using larger package sizes • Standard formulation General description Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth. Packaging 1 kg in poly bottle Reconstitution Add 35. Boeing 737 900er Fsx Download Game. 6g powder in 1L water.

Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Application Suitable for non-selective cultivation of E. Dwg Xls Converter there. Coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin ®. Legal Information EZMix is a trademark of Sigma-Aldrich Co. LLC Zeocin is a registered trademark of Cayla Sarl. Each of the broths will likely grow E. Coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol.

Generally: LB - Miller and LB - Lennox are used for E. Coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics. LB - Luria low salt is used for special applications where the E.

Coli growth or other constraints require the lowest possible salt content. Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E.

Coli in this medium. SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells. SOC is used for initial growth of competent cells and the transformation procedure.