User Guide Centricon. With or arising from the use of this manual. BIOSEPARATIONS ® Millipore, Amicon, Centricon and Ultracel are registered. Amicon Ultra-15 Centrifugal Filter Units. Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization. Pressure-based sample concentration Find MSDS or SDS, a COA, data sheets and more information.

Documentation Brochure Title Posters Title References Reference overview Application Simultaneous reduction and alkylation of protein disulfides in a centrifugal ultrafiltration device prior to two-dimensional gel electrophoresis. Smejkal GB, Li C, Robinson MH, Lazarev AV, Lawrence NP, Chernokalskaya E. J Proteome Res. 2006 Apr;5(4):983-7. 2006 Protein Concentration Ultrafiltration for proteomic sample preparation E. Chernokalskaya, S. Gutierrez, A.M. Tehran Street Map Pdf.

Leonard Electrophoresis, 25: 2461-2468 2004 Sample Prep FAQ Question Answer Can the Amicon Ultra be spun to dryness? No, the Amicon Ultra has a built in deadstop. What is the maximum sample volume for Amicon Ultra? Amicon Ultra-4 Amicon Ultra-15 Swinging Bucket 4ml 15ml Fixed Angle 4ml(35 & 45 degree rotor angle) 12ml(35 degree rotor angle) 3.5ml(23 degree rotor angle) Can Amicon Ultra units be sterilized with ethanol? Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization. Do you have any centrifugal filter units that are pyrogen free?

English Grammar Learning Games. We do not have any centrifugals devices that we claim are pyrogen free. Even the sterile devices could contain pyrogens.

Amicon Ultra units are offered in what volumes? Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.

What is the recommended rotor for Amicon Ultra units? Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units. Can the Amicon Ultra be used with nucleic acids? Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time.

Protocols related to Genomic and PCR fragments can be found in the user guide. What is the maximum centrifugal force I can spin Amicon Ultra-4 & Amicon Ultra-15? 4ml 15ml Swinging Bucket 4000 x g 4000 x g 3500 x g for concentrated/viscous solutions Fixed Angle(23, 35 &45 degrees) 7500 x g 5000 x g What is the chemical compatibility of Amicon Ultra?

Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support. How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra? Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.